Isolation of biosurfactant producing bacteria from. The e24 of all three organisms are noted in table 3. Isolation of biosurfactant producing bacteria from oil reservoirs. Screening, isolation and characterization of biosurfactant producing. In light of this, the work embarked with an attempt to isolate potential biosurfactant producing bacteria from various processes involved in the milling of. Present study focused on biosurfectant producing bacteria from lonar lake. View the article pdf and any associated supplements and figures for a period of 48 hours. Biosurfactant from microorganisms was proven to be an alternative source over chemical surfactant due to its structural and functional similarity and biodegradability properties. The biosurfactant producing ability of bacteria was investigated by oil displacement agar plate and emulsification index method. Accordingly, the aims of this study were to isolate and characterize of novel biosurfactant producing bacteria isolated from palm oil contaminated soils in palm oil industry. Among 64 bacterial strains isolated in this study, the best two of biosurfactantproducing bacteria were selected and identified based on the phenotypic properties and molecular approach based on. Isolation and characterization of biosurfactantproducing. Isolation and enrichment of biosurfactant producing bacteria two biosurfactant producing bacterial strains were isolated and enriched from diesel oil contaminated soil in dindigul 10.
Most biosurfactant producing microorganisms reported are from the genus pseudomonas, candida and bacillus is known to produce rhamnolipids, glycolipids and lipopeptide surfactants 3, 11. All of the bacterial isolates were grown in mineral salt medium msm with addition of 1% vv tapis crude oil as carbon source. Isolation and screening of biosurfactant producing bacteria. Occurrence of biosurfactantproducing bacteria in hydrocarbonpolluted environments was reported by many researchers yateem et al. Haemolysis has been used as an initial selection criterion for the primary isolation of surfactant producing bacteria. Isolated microorganisms were identified based on the biochemical characterization as. Abstract biosurfactants are amphiphilic compounds secreted extracellularly by microorganisms. Isolation of biosurfactant producing bacteria from petroleum. The growth optimization of halomonas sp bs4 revealed their optimum growth at 8% nacl and 68 ph in the growth medium. The values are meaning of three readings, emulsification index 30% is indicated in bold to show high activity. Original article characterization and phylogenetic analysis of biosurfactantproducing bacteria isolated from palm oil contaminated soils kanokrat saisaard1, atipan saimmai2,3, and suppasil maneerat4 1 faculty of science and technology, suratthani rajabhat university, mueang, surat thani, 84100 thailand. Alternative methodology for isolation of biosurfactant.
Antibiotic sensitivity test for biosurfactant producing bacteria 8. In this study, isolation and identification of biosurfactant producing bacteria were assessed. Biosurfactant plays an important role in bioremediation of crude oil contamination and microbial enhanced oil recovery meor. In recent time, biosurfactants produced by diverse group of microorganisms are. Nov 11, 2016 affandi ie, suratman nh, abdullah s, ahmad wa, zakaria za 2014 degradation of oil and grease from highstrength industrial effluents using locally isolated aerobic biosurfactant producing bacteria. Here, crude oil was used as the hydrophobic substrate. Isolation of biosurfactant producing bacteria from garbage. Bacteria are the main group of biosurfactantproducing microorganisms, although it is also produced by some yeast and filamentous fungi.
The oil contaminated soil samples were collected from varachha, surat. Higher concentrations of biosurfactant led to increase in the diameter of zone of inhibition. Isolation of biosurfactant producing bacteria from garbage soil. A number of microorganisms have been stated to produce a number of classes of. Physical characterization of bacterial isolates 11.
Only 37 of 492 strains of different these 37 strains, together with 49 nonhaemolytic ones chosen at random, were studied for surface activity. L1 showed it was useful in terms of screening and maintenance of biosurfactantproducing bacteria from a highly eutrophic environment. And in resulting wide variety of biosurfactant can be produced accordingly to the demand and uses. Isolation, characterization and antibacterial effect of. Emulsification assay is an indirect method used to screen biosurfactant production. In well diffusion assay, biosurfactant produced a zone of inhibition against s. Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. Distribution of biosurfactantproducing bacteria in. Among 64 bacterial strains isolated in this study, the best two of biosurfactant producing bacteria were selected and identified based on the phenotypic properties and molecular approach based on.
Vijaya,et at 20isolated 42 isolates and selected for biosurfactant production. In the present study, a biosurfactant producing candida parapsilosis strain was isolated and identified by our laboratory. The direct isolation of the microorganisms was carried out using serial dilution up to 10. These compounds can be synthesized by microorganisms growing on waterimmiscible hydrocarbons, as well as on watersoluble compounds such as glucose, sucrose, glycerol, or ethanol, and can be excreted or. Total 30 bacterial strains were isolated from samples of soil contaminated with petroleum and its products. Bacterial quantification bq quantification was performed following carlucci et al. Crude oil is a highly hydrophobic material with most of its components having low water solubility kuyukina et al. Biosurfactants or surfaceactive compounds are produced by microoaganisms. Characterization and phylogenetic analysis of biosurfactant. Different biosurfactant screening tests such as drop collapse, oil spreading, emulsification index and hemolytic activity confirmed the production of biosurfactant by the isolated candida parapsilosis strain. The search for biosurfactantproducing microorganisms in the pinyon plant jatropha curcas l. Biosurfactant production was assessed by such parameters as drop collapse, haemolysis, emulsification and oil spreading test. During the production of biosurfactants their growth in the culture medium was assessed by optical density at the wavelength of 600nm. Biosurfactantproducing bacteria were found to constitute a significant.
Fortyfive strains of bacteria, comprising 18 acinetobacter and 27 other bacteria along with positive mtcc reference strains were examined. Only 37 of 492 strains of different origins had haemolytic activity. These 37 strains, together with 49 nonhaemolytic ones chosen at random, were studied for surface activity. Occurrence of biosurfactant producing bacteria in hydrocarbonpolluted environments was reported by many researchers yateem et al. Research article isolation, screening and characterization. Bacteria are the main group of biosurfactant producing microorganisms, although it is also produced by some yeast and filamentous fungi. The combination of oildegrading bacteria and biosurfactant or biosurfactantproducing bacteria has also been tested by research groups. Isolation and screening of biosurfactant producing bacteria from oil contaminated soils in iraq doi. Screening concepts for the isolation of biosurfactant.
The strain4 biosurfactant component was mainly glycolipid in nature. In addition, lowcost substrates for biosurfactant production were evaluated. Haemolysis has been used as an initial selection criterion for the primary isolation of surfactantproducing bacteria. The combination of oildegrading bacteria and biosurfactant or biosurfactant producing bacteria has also been tested by research groups. Biosurfactants are amphiphilic compounds secreted extracellularly by microorganisms. Ruwaida kuwait institute of scientific research, p.
These molecules reduce surface tension both aqueous solutions and hydrocarbon mixtures. Ghayyomi et al 2012 isolated a strain of bacillus sp. Surfactants are chemicals that change the properties of water and other fluids. Apr 10, 20 halophilic bacteria were isolated from thamaraikulam solar salt works in india. Isolation and characterization of biosurfactant production under. Soils were screened for biosurfactantproducing isolates by using the following procedure. Screening of biosurfactant production was performed using the. The goal of this study was isolation and characterization of biosurfactant producing bacteria from caspian sea water. Ten morphologically distinct microbial colonies were isolated and.
This strain is potential candidate for microbial enhanced oil recovery. Characterization, antimicrobial and metal remediation properties. A 5g sample of each soil was placed into a 250ml flask containing 50 ml of tap water and incubated at 23c on a shaker at 200 rpm for 21 days. When oil spread in the environment, most of the oil hydrocarbons remain. After screening, candidate biosurfactantproducing bacteria were selected for optimization of the physical and. After extraction and purification of the biosurfactant produced by the diculture, it was found that the biosurfactant contained 61% carbohydrate, 30% lipid and 9% protein and was classified as a carbohydrate protein lipid complex. The annual release of crude oil in the oceans is estimated to be around 1. Isolation of biosurfactant producing bacteria from oil. Only five strains, all of them haemolytic, tested positive. Ten out of thirty strains showed the biosurfactant activity. Bacteria are the main group of biosurfactant producing microorganisms, although they are also produced by some yeasts and filamentous fungi.
Nov 01, 2002 culturable screening for biosurfactantproducing isolates. Kleber encyclopedia of life support systems eolss biosurfactant producing organism csource localization1 charge surface tension mn m1 cmc mg l1 interfacial tension mn m1 cellobioselipids ustilago sp. Research projects seeking funding isolation and characterization of biosurfactant producing bacteria from oil polluted environment. It was presumed that if the cell free culture broth contains biosurfactant then it would emulsify the hydrocarbons present. Isolation of biosurfactant producing exiguobacterium sp. Screening and identification of biosurfactant bioemulsifier producing bacteria from crude oil contaminated sites of gujarat, india. Screening and optimization of biosurfactant production by. Sixty nine hydrocarbon utilizing bacteria isolated from selected crude oil contaminated sites of ongc gujarat were screened for biosurfactant producing potential. Many biosurfactant producing microbes were isolated from soils or water samples which are contaminated with hydrophobic organic compounds like e. The presence of biosurfactant was determined by the dropcollapse test, microplate analysis, oil spreading technique, emulsification index %ei 24 and surface tension. Isolation of isolation of biosurfactant producing bacteria from oil reservoirs a tabatabaee 1, m mazaheri assadi 2, a a noohi 1,v a sajadian 3 1faculty of science, research and technology, islamic azad university, iran 2 biotechnology center of iranian research organization for science and technology, iran 3research institute of petroleum industry, tehran, iran.
Guanabara bay and its mangrove protection areas are constantly subject to a multiple source of petroleum. Research article isolation, screening and characterization of. Distribution of biosurfactant producing bacteria was abundant in various season water and sediment. Biosurfactant producing bacterial density was observed for a period of one year december 2009 to november 2010. Pdf isolation of biosurfactant producing bacteria from. In light of this, the work embarked with an attempt to isolate potential biosurfactantproducing bacteria from various processes involved in the milling of. Isolation of biosurfactant producing bacteria braz. Identification of biosurfactant producing bacteria.
They were present in water and sediment samples respectively in the density of 10 2 and 10 3 cfumlg. A number of studies have reported the potential of bacillus species as biosurfactant producers and they produce lipopeptide type of biosurfactant nitschke and pastore, 2004. Biosurfactant producing bacteria, biosurfactant, emulsification, microbial enhanced oil. Assessment of different screening methods for selecting. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. Physiological and molecular characterization of biosurfactant.
The highest biosurfactant production excreted and cellbound biosurfactants was achieved with lactobacillus paracasei ssp. Biosurfactant production was screened in four lactobacilli strains. Most biosurfactantproducing microorganisms reported are from the genus pseudomonas, candida and bacillus is known to produce rhamnolipids, glycolipids and lipopeptide surfactants 3, 11. Biosurfactants are produced by a variety of microorganisms mainly bacteria, fungi and yeasts are diverse in chemical composition and their nature and the amount depend on the type of microbes producing a particular biosurfactant. The word surfactant is derived from the term surface active agent. One biosurfactantproducing bacteria achromobacter sp. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, halomonas sp bs4 was confirmed by 16 s rrna sequencing. Qualitative analysis of biosurfactant production was identified by performing assays like hemolysis on blood agar, penetration assay and ctab. After screening, candidate biosurfactant producing bacteria were selected for optimization of the physical and. Affandi ie, suratman nh, abdullah s, ahmad wa, zakaria za 2014 degradation of oil and grease from highstrength industrial effluents using locally isolated aerobic biosurfactantproducing bacteria. Isolation and selection of biosurfactantproducing bacteria. Ten selected biosurfactant producing strains were screened for biosurfactant production by numerous tests. Pdf isolation of biosurfactant producing bacteria from oil. Two bacterial strains were isolated having ability to produce biosurfactant.
The biosurfactant showed significant emulsifying index, drop. Ha, mdc, tgst efficiently detected 15, 17 and 14 biosurfactant producers respectively. Isolation and screening of biosurfactant producing. The potential application of these bacteria in petroleum industry was investigated. Considering leakage of crude oil from tankers in harbors and other anthropogenic activities, we suspected the occurrence of biosurfactantproducing bacteria at our sampling site ebrahimi et al. Characterization of bacteria isolation of bacteria from. Screening and identification of biosurfactant bioemulsifier. According to these physiological roles, biosurfactant producing microbes can be found in different environments. From four different sites of lonar lake 12 sediment, matt and water samples were collected and screened on mineral salt medium containing 2% soybean oil as a carbon source. The isolates showed an exponential growth in the medium. The present study aimed the isolation of a potent biosurfactant producing indigenous bacteria which can be employed for crude oil remediation. Culturable screening for biosurfactantproducing isolates.
Screening, isolation and characterization of biosurfactant. The biosurfactant producing bacteria were isolated from oil contaminated site. Isolation and characterization of biosurfactant producing. Screening and identification of biosurfactant bioemulsifier producing bacteria from crude oil contaminated sites of gujarat, india s. Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated.